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Anti-nitro-Tyrosine mouse mAb

  • 服务简介

    Product Description

    The mouse-derived anti-3NT antibody is purified with Protein G. It specifically recognizes proteins with tyrosine nitrated residues but not the tyrosine phosphorylated or non-modified tyrosine residues. Besides common applications in Western blotting and immunoprecipitation, the antibody has been well utilized for proteomic screening to tyrosine nitrated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.


    Source/Purification

    This product is produced by immunizing mice with nitrated KLH。 The ascites was purified by protein G。


    Specificity

    All nitrated proteins expected

    Recommended Applications

    ELISA, WB, IP   

    *WB=Western blotting;  IP=Immunoprecipitation


    Recommended antibody dilution

    WB: 1:1000

    Important

    For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.

    Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.

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    Dot blotting analysis on indicated amount of peptides using 3-nitrotyrosine mouse mAb. The list of peptides is included in the table on the right. 

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    Western blot analysis on 30 μg of extracts from HeLa and MEF whole cell lysates, untreated or treated with peroxynitrite(3 mM, 30 mins) or pervanadate(3 mM, 30 mins), using anti-3-nitrotyrosine mouse Antibody (Figure B) or anti-phospho-Tyrosine rabbit pAb (PTM-702) (Figure C).


    Scientific Description

    Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide。 It is very stable and so is suitable for analysis in biological sample such as plasma, lung aspirants-BALF (Broncho alveolar lining fluid) and urine。 Increased nitrotyrosine levels have been associated with several physiological and pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress。 Research shows that nitrotyrosine levels can be reduced by N-acetyl cysteine which is a precursor to glutathione, one of the body's primary endogenous antioxidants。



    Storage & Stability

    The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage. 


    **For research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


  • 技术原理

    Cat#:

    PTM-752

    Ab Type:

    M

    Applications:

    WB

    Species:

    All

    Price: (100 μl)

    2880。00

    Price: (20 μl)

    864.00


  • 仪器设备

  • 技术优势

  • 应用领域

    ELISA, WB, IP   

    *WB=Western blotting;  IP=Immunoprecipitation


  • 参考文献

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