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Anti-acetyllysine antibody conjugated agarose beads

  • 服务简介

    Product Description

    mixture of high quality rabbit-derived polyclonal and mouse-derived monoclonal anti-acetyllysine antibodies are cross-linked to beaded agarose with stable amide linkages. With the combinative advantages of polyclonal and monoclonal antibodies in globe proteomic screening of lysine acetylation, the product presents extraordinary capability to specifically capture the peptides/proteins bearing lysine acetylated residues.


    Source/Purification

    /


    Specificity

    All

    Recommended Applications

    Protein/peptide immunoprecipitation for lysine acetylation proteomics; Immuno-affinity chromatography


    Recommended antibody dilution

    /

    Important

    Anti-acetyllysine antibody beaded agarose, Premium 

    Scientific Description

    Lysine acetylation is an important reversible modification controlling protein activity。 The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression。 Therefore, systematical screening of lysine acetylated substrates in various cellular pathological and physiological conditions is a key mission in lysine biology。 To address this, the affinity chromatography with anti-acetyllysine antibody beaded agarose followed by MS/MS identification is viewed as the most preferable and efficient method。


    Storage & Stability

    Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


    **Research purposes only.Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



  • 技术原理

    Cat#:

    PTM-104

    Ab Type:

    P/M

    Applications:

    IAC IP

    Species:

    All

    Price: (0。5 ml)

    22432.00

    Price: (0.1 ml)

     7800.00


  • 仪器设备

  • 技术优势

    Chen Y,et al.SILAC-based quantification of Sirt1-responsive lysine acetylome.Methods Mol Biol. 2013;1077:105-20. doi: 10.1007/978-1-62703-637-5_7. Li X,et al.

    Systematic identification of the lysine succinylation in the protozoan parasite Toxoplasma gondii。J Proteome Res。 2014 Dec 5;13(12):6087-95。 doi: 10。1021/pr500992r。 Epub 2014 Nov 17。 Huang D,et al。

    Lysine acetylproteome analysis suggests its roles in primary and secondary metabolism in Saccharopolyspora erythraea.Appl Microbiol Biotechnol. 2015 Feb;99(3):1399-413. doi: 10.1007/s00253-014-6144-2. Epub 2014 Dec 10. Ouidir T,et al.

    Proteomic profiling of lysine acetylation in Pseudomonas aeruginosa reveals the diversity of acetylated proteins.Proteomics. 2015 Jul;15(13):2152-7. doi: 10.1002/pmic.201500056. Epub 2015 May 26. Fang X,et al.

    Global analysis of lysine acetylation in strawberry leaves.Front Plant Sci. 2015 Sep 15;6:739. doi: 10.3389/fpls.2015.00739. eCollection 2015. Cluntun AA,et al.

    The rate of glycolysis quantitatively mediates specific histone acetylation sites.Cancer Metab. 2015 Sep 23;3:10. doi: 10.1186/s40170-015-0135-3. eCollection 2015. He D,et al.

    Global Proteome Analyses of Lysine Acetylation and Succinylation Reveal the Widespread Involvement of both Modification in Metabolism in the Embryo of Germinating Rice Seed.J Proteome Res. 2016 Mar 4;15(3):879-90. doi: 10.1021/acs.jproteome.5b00805. Epub 2016 Jan 28. Zhu X1,et al.

    Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells。Sci Rep。 2016 Jan 29;6:19926。 doi: 10。1038/srep19926。 Xiong Y,et al。

    A comprehensive catalog of the lysine-acetylation targets in rice (Oryza sativa) based on proteomic analyses.J Proteomics. 2016 Apr 14;138:20-9. doi: 10.1016/j.jprot.2016.01.019. Epub 2016 Feb 1. Zhou X,et al.

    Systematic Analysis of the Lysine Acetylome in Candida albicans。J Proteome Res。 2016 Aug 5;15(8):2525-36。 doi: 10。1021/acs。jproteome。6b00052。 Epub 2016 Jun 28。 Chen Y,et al。

    Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways. Mol Cell Proteomics. 2012 Oct;11(10):1048-62. Epub 2012 Jul 23. Pan J, et al.

    Systematic analysis of the lysine acetylome in Vibrio parahemolyticus. J Proteome Res. 2014 Jul 3;13(7):3294-302. doi: 10.1021/pr500133t. Epub 2014 Jun 9. Liu X,et al.

    Human borna disease virus infection impacts host proteome and histone lysine acetylation in human oligodendroglia cells.Virology. 2014 Sep;464-465:196-205. doi: 10.1016/j.virol.2014.06.040. Epub 2014 Aug 1. Xie L,et al.

    Proteome-wide lysine acetylation profiling of the human pathogen Mycobacterium tuberculosis. Int J Biochem Cell Biol. 2015 Feb;59:193-202. doi: 10.1016/j.biocel.2014.11.010. Epub 2014 Nov 29. Mo R,et al.

    Acetylome analysis reveals the involvement of lysine acetylation in photosynthesis and carbon metabolism in the model cyanobacterium Synechocystis sp。 PCC 6803。J Proteome Res。 2015 Feb 6;14(2):1275-86。 doi: 10。1021/pr501275a。 Epub 2015 Jan 26。 Nie Z,et al。

    Comprehensive profiling of lysine acetylation suggests the widespread function is regulated by protein acetylation in the silkworm, Bombyx mori。 Proteomics。 2015 Sep;15(18):3253-66。 doi: 10。1002/pmic。201500001。 Epub 2015 Jul 2。 Xie L,et al。

    Global profiling of lysine acetylation in human histoplasmosis pathogen Histoplasma capsulatum.Int J Biochem Cell Biol. 2016 Apr;73:1-10. doi: 10.1016/j.biocel.2016.01.008. Epub 2016 Jan 19. Xiong Y,et al.

    Data for global lysine-acetylation analysis in rice (Oryza sativa). Data Brief. 2016 Feb 20;7:411-7. doi: 10.1016/j.dib.2016.02.032. eCollection 2016 Jun. Liu L,et al.

    Acetylome analysis reveals the involvement of lysine acetylation in biosynthesis of antibiotics in Bacillus amyloliquefaciens.Sci Rep. 2016 Jan 29;6:20108. doi: 10.1038/srep20108. Shen Z,et al.

    Global-scale profiling of differential expressed lysine acetylated proteins in colorectal cancer tumors and paired liver metastases。 J Proteomics。 2016 Jun 16;142:24-32。 doi: 10。1016/j。jprot。2016。05。002。 Epub 2016 May 10 Meng Q,et al。

    Systematic analysis of the lysine acetylome of the pathogenic bacterium Spiroplasma eriocheiris reveals acetylated proteins related to metabolism and helical structure. J Proteomics. 2016 Oct 4;148:159-69. doi: 10.1016/j.jprot.2016.08.001. Epub 2016 Aug 4. Zhu D,et al.

    Crosstalk among proteome, acetylome and succinylome in colon cancer HCT116 cell treated with sodium dichloroacetate. Sci Rep. 2016 Nov 22;6:37478. doi: 10.1038/srep37478. Gu X,et al.

    Proteome and Acetylome Analysis Identifies Novel Pathways and Targets Regulated by Perifosine in Neuroblastoma。Sci Rep。 2017 Feb 6;7:42062。 doi: 10。1038/srep42062。 Song L,et al。

    Reversible acetylation on Lys501 regulates the activity of RNase II。Nucleic Acids Res。 2016 Mar 18;44(5):1979-88。 doi: 10。1093/nar/gkw053。 Epub 2016 Feb 4。


  • 应用领域

    Protein/peptide immunoprecipitation for lysine acetylation proteomics; Immuno-affinity chromatography

  • 参考文献

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