修饰抗体 Products
组蛋白赖氨酸单甲基化(mono-methylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-mono-methyl-Histone H2B (Lys5) rabbit pAb

Cat#:

PTM-678

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060。00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by mono-methylated histone H2B (Lys5) peptide affinity chromatography. It specifically recognizes histone H2B with mono-methylation at Lys5.  


Source/Purification

This product is produced by immunizing rabbits with a synthetic mono-methyl peptide corresponding to residues surrounding Lys5 of human histone H2B. Antibodies are purified by protein A-conjugated agarose followed by mono-methylated histone H2B (Lys5) peptide affinity chromatography.  


Specificity

H2B

Recommended Applications

ELISA,WB; Not tested in other applications 

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

 For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0。1% Tween-20 for two hours at room temperature with gentle shaking。  

 Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. 

 Anti-mono-methyl-Histone H2B (Lys5) rabbit pAb 

A。Dot  blotting analysis on indicated amount ofmono-methylated H2B peptide at Lys5  (lane 1), acetylatedH2B peptide at Lys5 (lane 2), butyrylatedH2B  peptide at Lys5 (lane 3), crotonylatedH2B peptide at Lys5 (lane 4), 2ohibutyrylatedH2B  peptide at Lys5 (lane 5), 3ohbutyrylatedH2B peptide at Lys5 (lane 6), crotonylatedH2B  peptide at Lys11 (lane 7), 3ohbutyrylatedH2B peptide at Lys11 (lane 8), acetylatedH2B peptide at Lys12 (lane 9), crotonylatedH2B peptide at Lys12  (lane 10),2ohibutyrylatedH2B peptide at Lys5 (lane 11), 3ohbutyrylatedH2B  peptide at Lys5 (lane 12)and unmodified H2B peptide at Lys5 (lane 13)using  mono-methyl-histone H2B (Lys5)rabbit pAb.


B.Western blotting analysis on 30 μg of crude proteins from H1299, MCF-7,  C2C12whole cell lysates and 200 ng recombinant histone H2B proteins using mono-methyl-histone  H2B (Lys5)rabbit pAb(1:2000)。  

 

Scientific Description

The ε-amino lysine methylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been associated with transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.

 

**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


ELISA,WB; Not tested in other applications 

*WB=Western blotting


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