修饰抗体 Products
酪氨酸磷酸化 (phosphorylation) 抗体
杭州景杰生物科技有限公司
Anti-phosphotyrosine rabbit pAb

Cat#:

PTM-702

Ab Type:

P

Applications:

WB

Species:

All

Price: (100 μl)

2880.00

Price: (20 μl)

864。00


Product Description

Anti-phosphotyrosine rabbit polyclonal antibody was developed by using phosphotyramine-KLH conjugates as immunogen. This antibody can be used in western blotting to examine the modification of tyrosine phosphorylation in immunoprecipitated proteins or the overall profile of tyrosine phosphorylation in cells and tissues.  


Source/Purification

The rabbit polyclonal antibody is produced by immunizing animals with synthetic phospho-Tyr-containing peptides and immunoaffinity purified by protein A followed by phosphotyramine affinity chromatography.  


Specificity

All

Recommended Applications

ELISA, WB, IAP。 Not tested in other applications

*WB=Western blotting; IAP: Immuno affinity precipitation


Recommended antibody dilution

WB: 1:1000; IAP: 1:200

Important

For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.

Anti-phosphotyrosine rabbit pAb

A。 Dot blotting analysis on 20 ng of tyrosine phosphorylated peptide library (1A), site specific tyrosine phosphorylated peptides (2A-5A,1B-5B and 1C-4C), threonine phosphorylated peptides (5C and 6A-6C), serine phosphorylated peptides (7A-7C and 8A) and unmodified peptides (8B-8C) using anti-phosphotyrosine rabbit polyclonal antibody . 

B. Western blotting analysis on 30 µg of crude proteins extracted from HeLa cells with (right) or without (left) sodium vanadate treatment (1 mM for 15 mins) using anti-phosphotyrosine rabbit polyclonal antibody (1:1000).

 

Scientific Description

Tyrosine phosphorylation is one of the major means of cell signaling transduction in multiple pathophysiological conditions regulated by tyrosine kinase activities. In cancer, unregulated tyrosine kinase activity can drive tumor malignancy and formation by generating inappropriate proliferation and survival signals. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to these studies. The anti-phosphotyrosine rabbit polyclonal antibody developed by PTM Biolabs, Inc. provides an exceptionally sensitive tool for studying tyrosine phosphorylation and its kinase activities in biomedical and preclinic studies as well as high throughput drug discovery.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ELISA, WB, IAP. Not tested in other applications

*WB=Western blotting; IAP: Immuno affinity precipitation


推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据。。。

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据。。。

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!
广东11选5 时时彩计划 广东11选5 中福网秒速赛车 广东11选5 广东快乐十分人工计划 广东快乐十分人工计划 广东11选5 广东11选5 手机时时彩网站