修饰抗体 Products
赖氨酸巴豆酰化 (crotonylation)抗体
杭州景杰生物科技有限公司
Anti-crotonyllysine mouse mAb (clone 4D5)

Cat#:

PTM-502

Ab Type:

M

Applications:

WB

Species:

All

Price: (100 μl)

3360.00

Price: (20 μl)

1008.00


Product Description

The pan anti-crotonyllysine monoclonal antibody (clone 4D5) is developed by using crotonylated-KLH conjugates as immunogen。 The product specifically recognizes proteins with lysine crotonylated residues but not the structurally-similar modified lysine residues or non-modified lysine residues。 Besides common applications in Western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry, the antibody is with ChIP grade quality and has been well utilized for proteomic screening to lysine crotonylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells。  


Source/Purification

The pan anti-crotonyllysine monoclonal antibody (clone 4D5) is developed by using crotonylated-KLH conjugates as immunogen.


Specificity

All crotonylated proteins expected

Recommended Applications

ELISA, WB, IAP. Not tested in other applications

*WB=Western blotting; IAP: Immuno affinity precipitation


Recommended antibody dilution

WB: 1:1000; IAP: 1:200

Important

For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0。1% Tween-20 at 4℃ with gentle shaking, overnight。

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.

Anti-crotonyllysine mouse mAb (clone 4D5)

A. Dot blotting analysis on 10 ng of lysine crotonylated peptide library (A1), lysine crotonylated BSA (B1), selected histone lysine crotonylated peptides (A2-7, B2-6, C1-6), unmodified lysine peptide library (B7), unmodified BSA (C7), lysine acetylated peptide library (A8), lysine propionylated peptide library (B8), lysine butyrylated peptide library (C8) and randomly selected histone unmodified peptides (A9, B9, C9) using anti-crotonyllysine mAb (clone 4D5, 1:1000).


B. Western blotting analysis on 30 μg of HeLa crude proteins using anti-crotonyllysine mAb (clone 4D5, 1:1000).


Scientific Description

Lysine crotonylation is a newly-identified histone post-translational modification by integrated proteomic approaches and elaborate biochemistry analyses。 It has been shown that lysine crotonylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes in a wide range of proteins, both in histones and non-histone substrates。 The unique structure and genomic localization of lysine crotonylation suggest that it is mechanistically and functionally different from lysine acetylation。 Given the revealed roles of lysine crotonylation in spermatogenesis, the novel modification may play important roles in multiple cellular pathophysiological processes by effecting chromatin structure and epigenetic pathways。


Storage & Stability

Store product at -20oC。 Avoid repeated freeze/thaw。 Antibody is supplied in PBS with 50% glycerol and 0。01% sodium azide。Stable for 12 months from date of receipt。


**Research purposes only。 Not intended to be used for therapeutic or diagnostic purposes in humans or animals。



  Tan, M.J. et al (2011) Cell 146 ,1016

ELISA, WB, IAP. Not tested in other applications

*WB=Western blotting; IAP: Immuno affinity precipitation


推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据。。。

等待加载动态数据...

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据。。。

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!
秒速赛车免费计划 广东11选5 时时彩平台 广东11选5 广东快乐十分开奖记录 手机时时彩网站 极速3d 秒速赛车精准计划 广东11选5 广东11选5