修饰抗体 Products
赖氨酸乙酰化 (acetylation) 抗体
杭州景杰生物科技有限公司
Anti-acetyllysine antibody (clone Kac-11)

Cat#:

PTM-102

Ab Type:

M

Applications:

WB

Species:

All

Price: (100 μl)

2580.00

Price: (20 μl)

774.00


Product Description

The mouse monoclonal pan anti-acetyllysine antibody (clone Kac-11) is purified with Protein G affinity chromatography. It specifically recognizes proteins with acetyllysine residues but not the propionyllysine or butyryllysine residues with structural similarity. Though the antibody shares the similar recognition pattern with anti-acetyllysine antibody (Cat#PTM-101, clone Kac-01), it has also its preferably detective profile to some acetylated substrates. Except the applications in Western blotting, immunoprecipitation and immunofluorescence, the antibody has been shown in combination with anti-acetyllysine antibody (Cat#PTM-101, clone Kac-01) to identify most lysine acetylated peptides in both prokaryotic and eukaryotic cells in proteomics screenings.


Source/Purification

The mouse monoclonal antibody is produced by immunizing animals with acetylated BSA. Antibodies are purified by acetyllysine-conjugated agarose from media of cultured cells.  


Specificity

All

Recommended Applications

 ELISA, WB, IP 

 *WB=Western blotting; IP=Immunopreciptation


Recommended antibody dilution

WB: 1:1000; IP: 1:200

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 

Anti-acetyllysine antibody (clone Kac-11)

 

 

Scientific Description

The post-translational ε-amino lysine acetylation of proteins, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of protein acetylation status is impaired in the pathologies of cancer and other diseases. HDACs have become promising targets for anti-cancer drugs.


Storage & Stability

Store product at -20oC。 Avoid repeated freeze/thaw。 Antibody is supplied in PBS with 50% glycerol and 0。01% sodium azide。Stable for 12 months from date of receipt。


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



Global Proteome Analyses of Lysine Acetylation and Succinylation Reveal the Widespread Involvement of both Modification in Metabolism in the Embryo of Germinating Rice Seed. J Proteome Res. 2016 Mar 4;15(3):879-90. doi: 10.1021/acs.jproteome.5b00805. Epub 2016 Jan 28.

 ELISA, WB, IP 

 *WB=Western blotting; IP=Immunopreciptation


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